Evaluating impact of possible transgenic poplar cultivation on protected areas

Background Plant biodiversity studies have been performed in the Migliarino-San Rossore-Massaciuccoli Regional Park in Tuscany (Italy) within the framework of the European project LIFE08 NAT/IT/342.This project aims at developing a quick monitoring index (QMI) to rapidly assess the potential risk generated by transgenic plants in characterized ecosystems or biotopes. For this reason test areas have been selected inside the protected area to evaluate plant (weeds and trees), animal, and soil microoganisms biodiversity. The proximity of the selected test area to cropped surfaces where Genetically Modified Plants (GMPs) might be cultivated has been taken into account. GMPs could spread pollen and contaminate natural populations. To avoid this risk, an efficient monitoring system is required taking into account genetic diversity and breeding study. As far as tree biodiversity concern, Populus species were identified in the test areas. Two populations of Populus present into two different ecosystems (forest and wetland areas) were examined together with two cultivated varieties. The two ecosystems were characterized for the vegetation. Nuclear microsatellites were used to evaluate genetic diversity of poplar populations and level of breeding between natural and cultivated Populus. In addition the insect populations present on male and female poplars during flowering period have been studied.